Mu.Ta.Lig - COST ACTION CA15135


14 June 2016


General information

Name:    Rona
Surname:  Ramsay
Cell phone number with international prefix: +44 1334 463411 (office)
Country:  UK
Affiliation:  University of St Andrews
Gender: F □ x
Year of the PhD title:  1977
Personal web page: http://
Previous COST participation: Yes □   D64 and CM1103


List of 10 selected publications within last 5 years

1. Nikolic,K., Lazaros Mavridis, Oscar M Bautista-Aguilera, Jose Marco-Contelles, Holger Stark, Maria do Carmo Carreiras, Ilaria Rossi, Paola Massarelli, Danica Agbaba, Rona R Ramsay, John B. O. Mitchell (2015) Predicting targets of compounds against neurological diseases using cheminformatic methodology. Journal of Computer-Aided Molecular Design 29 (2), 183-198. DOI: 10.1007/s10822-014-9816-1
2. Benek,O, Ondrej Soukup, Marketa Pasdiorova, Lukas Hroch, Vendula Sepsova, Petr Jost, Martina Hrabinova, Daniel Jun, Kamil Kuca, Dominykas Zala, R. R. Ramsay, José Marco-Contelles, Kamil Musilek. (2015) “Design, synthesis and in vitro evaluation of indolotacrine analogues as multi-target-directed ligands for the treatment of Alzheimer’s disease” ChemMedChem. DOI: 10.1002/cmdc.201500383
3.  Malcomson,T, Kemal Yelekci, Maria Teresa Borrello, A. Ganesan, Elena Semina, Norbert De Kimpe, Sven Mangelinckx, and Rona R. Ramsay (2015) cis-Cyclopropylamines as mechanism-based inhibitors of Monoamine Oxidases. FEBS J. 282 (16) 3190-3198 DOI: 10.1111/febs.13260
4.  Bautista-Aguilera,OM, Abdelouahid, Samadi, Mourad Chioua, Katarina Nikolic, Slavica Filipic, Danica Agbaba, Elena Soriano, Lucía de Andrés, María Isabel Rodríguez-Franco, Stefano Alcaro, Rona R. Ramsay, Francesco Ortuso, Matilde Yañez, and José Marco-Contelles (2014) N-Methyl-N-((1-methyl-5-(3-(1-(2-methylbenzyl)piperidin-4-yl)propoxy)-1H-indol-2-yl)methyl)prop-2-yn-1-amine, a New Cholinesterase and Monoamine Oxidase Dual Inhibitor. J. Med. Chem. 57 (24) 10455-10463. DOI: 10.1021/jm501501a
5. Messina,A, Simona Reina, Francesca Guarino, Andrea Magrì, Flora Tomasello, Richard E. Clark, Rona R. Ramsay and Vito De Pinto (2014) Live cell interactome of the human Voltage Dependent Anion Channel 3 (VDAC3) revealed in HeLa cells by Affinity Purification Tag Technique. Molecular BioSystems, 10 (2014) 2134-2145. DOI:10.1039/c4mb00237g
6. Esteban,G, Allan J, Samadi A, Romero A, Pino JD, Herreroa R, Martínez-Murilloa, R., Mattevi, A., Unzeta, M., Marco-Contelles, J., Binda, C., and Ramsay, R. R. (2014) Kinetic and structural analysis of the irreversible inhibition of human monoamine oxidases by the multi-target ASS234 compound and biological assessment for its potential use in Alzheimer’s disease. Biochim. Biophys. Acta (Proteins and Proteomics), 1844 (2014) 1104–1110.
7. Juárez-Jiménez,J., M. E. Mendes, C. Martins, D.B. Silva, J. Marco-Contelles, M. C. Carreiras, F.J. Luque and R.R. Ramsay (2014). Exploring the structural basis of the selective inhibition of monoamine oxidase A by dicarbonitrile aminoheterocycles: Role of Asn181 and Ile335 validated by spectroscopic and computational studies. Biochim. Biophys. Acta (Proteins and Proteomics), 1844: 389–397.
8. Ramsay,R.R.,(2013) Monoamine Oxidases: The Proteins as Targets in Medicinal Chemistry and Drug Discovery. Curr. Topics Med. Chem. 12: 2189-2209.
9. Ramsay,R.R., A. Olivieri, A. Holt (2011) An improved approach to steady-state analysis of monoamine oxidases. J. Neural Transm. 118:1003–1019. DOI 10.1007/s00702-011-0657-y
10. Dixon Clark,S.E. and R.R. Ramsay (2010) Dietary inhibitors of monoamine oxidase A. J. Neural Transm. 118:1031–1041. DOI 10.1007/s00702-010-0537-x.


Main skills and expertise (up to 5)

1. Enzymology
2. Protein purification
3. Mitochondrial bioenergetics
4.  UV-vis spectrocospy
5. Cell-based assays


Main equipment/facilities available in the participants’ lab (up to 5)

1. Spectrophotometers (UV-vis and stopped-flow)
2. Plate readers
3. Protein purification (centrifuges, fraction collectors etc)
4. Proteomics (access charge)
5. “Seahorse” bioenergetics analyzer (access charge)


Short personal activity proposal for the COST Action CA15135 (max 1000 characters)

The Ramsay group uses purified monoamine oxidase for chemical studies, applying biochemical techniques for kinetics and mechanism to validate theoretical work. Screening assays on a range of membrane bound enzymes are also available. We are currently pioneering a cell-based neuronal model for screening mitochondrially-targetted compounds and aim to run differential proteomics to identify factors underlying the effects of selected key proteins on cell survival.


Through COST Actions, I can share my expertise in biological assays, and  enjoy collaborations to integrate theory and experiment, and chemistry and biology.



Work Group preference: score from 1 (preferred) to 4 (not preferred)

Work Group of the CA15135 COST Action Score
WG1: Development of new chemical entities 3
WG2: Selection of biological targets and assessment of biological data 1
WG3: Development of chemical databases 4
WG4: Development of Computational methods for multiple ligand design and discovery 2